Functional domain mapping of peroxin Pex19p: interaction with Pex3p is essential for function and translocation
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چکیده
منابع مشابه
Functional domain mapping of peroxin Pex19p: interaction with Pex3p is essential for function and translocation.
The peroxin Pex19p functions in peroxisomal membrane assembly. Here we mapped functional domains of human Pex19p comprising 299 amino acids. Pex19p mutants deleted in the C-terminal CAAx farnesylation motif, the C-terminal 38 amino acid residues and the N-terminal 11 residues, maintained peroxisome-restoring activity in pex19 cells. The sequence 12-261 was essential for re-establishing peroxiso...
متن کاملPex19p interacts with Pex3p and Pex10p and is essential for peroxisome biogenesis in Pichia pastoris.
We report the cloning and characterization of Pichia pastoris PEX19 by complementation of a peroxisome-deficient mutant strain. Import of peroxisomal targeting signal 1- and 2-containing peroxisomal matrix proteins is defective in pex19 mutants. PEX19 encodes a hydrophilic 299-amino acid protein with sequence similarity to Saccharomyces cerevisiae Pex19p and human and Chinese hamster PxF, all f...
متن کاملStructural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p.
Peroxisomes require peroxin (Pex) proteins for their biogenesis. The interaction between Pex3p, which resides on the peroxisomal membrane, and Pex19p, which resides in the cytosol, is crucial for peroxisome formation and the post-translational targeting of peroxisomal membrane proteins (PMPs). It is not known how Pex3p promotes the specific interaction with Pex19p for the purpose of PMP translo...
متن کاملPeroxisome biogenesis in mammalian cells
To investigate peroxisome assembly and human peroxisome biogenesis disorders (PBDs) such as Zellweger syndrome, thirteen different complementation groups (CGs) of Chinese hamster ovary (CHO) cell mutants defective in peroxisome biogenesis have been isolated and established as a model research system. Successful gene-cloning studies by a forward genetic approach utilized a rapid functional compl...
متن کاملThe peroxin pex3p initiates membrane assembly in peroxisome biogenesis.
Rat cDNA encoding a 372-amino-acid peroxin was isolated, primarily by functional complementation screening, using a peroxisome-deficient Chinese hamster ovary cell mutant, ZPG208, of complementation group 17. The deduced primary sequence showed approximately 25% amino acid identity with the yeast Pex3p, thereby we termed this cDNA rat PEX3 (RnPEX3). Human and Chinese hamster Pex3p showed 96 and...
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ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 2006
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.03100